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Below is a link to my research paper written by me and fellow student Adam Housby
CPAC 9: Investigating factors affecting the rate of aerobic respiration using a respirometer12/10/2016 Apparatus:
Safety: Soda lime is highly corrosive so eye protection should be worn at all times and contact with the soda lime should be avoided. A spatula should be used to handle it. After handling the maggots you should wash your hand thoroughly. Procedure:
Results: As we can see from these results, overall as the temperature increased, so did the rate of respiration measured by the distance the indicator moved along the glass tube in a minute (measured in mm). However the optimum temperature seemed to be 40 degrees Celsius and after the point the rate of respiration began to decrease again until at 50 degrees Celsius it was at 0.
The rate of respiration actually reached 0 at 55 degrees Celsius because the temperature turned out to be too hot for the maggots and they perished under the conditions which was very unethical and for future experiments lower temperatures needs to be used to ensure the protection of the organisms. Reticulated pythons or python reticulatus are a species of snake found in Southern Asia and are part of the python genus meaning they are nonvenomous and kill there prey by constriction and asphyxiation. They are considered as the world's longest (although to the bulkiest) snake with the largest accurately recorded length to be of a snake known as Medusa who measured in at an incredible 7.67 m (25 ft 2 in) long [1] although there have been reports of snakes reaching 10.1 m (33 ft 1 in). These snakes have been suggested to be one of the few snakes who can actually target humans as prey and have been linked to a number of human fatalities. The Respiratory system Snakes have a small opening just behind the tongue called the glottis, which opens into the trachea. Unlike what mammals have, the reptile glottis is always closed, forming a vertical slit, unless the snake takes a breath. A small piece of cartilage just inside the glottis vibrates when the snake forcefully expels air from its lungs. This produces a snake’s characteristic hiss. Snakes are able to extend their glottis out the side of their mouth while they eat, which allows for respiration while they consume large prey items. The trachea is a long, straw-like structure supported by cartilaginous rings. These rings are incomplete in that the snake looks more like a C than an O. A thin membrane completes the open part of the C. This configuration is also seen in lizards, but the function of the incomplete rings remains unknown. The trachea usually terminates just in front of the heart, and at this point it splits into the two primary bronchi, airways that direct air into either the left or right lung. In most snakes the short left bronchus terminates into a rudimentary left lung and only the right lung is used. however in the case of Boas and Pythons such as our Reticulated Python both lungs are used. The left lung can also be used by water snakes but as an aid for buoyancy rather than for respiration. Snakes breathe principally by contracting muscles between their ribs. Unlike mammals, they lack a diaphragm. Inspiration is an active process (muscles contract), whereas expiration is passive (muscles relax). The portion of a snake’s lung nearest its head has a respiratory function; this is where oxygen exchange occurs. The lung portion nearest the tail, regardless of the lung’s size, is more of an air sac. The inside of these sac portions look more like the inside of a balloon than a lung. There is no exchange of respiratory gases.
Procedure:
Results: The image on the top left is the results I obtained from carrying out this experiment and the photo on the top right is a scale showing how the hydrogen carbonate indicator reacts to different pH levels. As we can see the glass vial that was left out without a colored filter around it turned a deep purple color and from the scale this suggest that the pH in that vial was around 8.5. This is because since all wavelengths of light were allowed into the vial so that the alga in their could photosynthesize to their full ability and the production of CO2 coming from that photosynthesis is what caused the change of pH within the vial.
However as we can see with the vial which had the green filter on, the color has stayed yellow suggesting a pH of around 7.9. This is because that the green filter was inhibited all wavelengths of light other than those in the green spectrum. As we can see on the below graph, the pigments within the chloroplasts responsible for photosynthesis are unable to absorb light in the green spectrum (around 520-550 nm) and since the alga cannot photosynthesis, CO2 isn't produced to alter the pH level of the solution like with the vial without the filter. |
Jack BojanArdingly College Archives
March 2017
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