Method: 1. Prepare a broth inoculated with yeast bacteria. 2. Using a control of 1 part uninoculated broth mixed with 9 parts water, set the colorimetre. 3. Prepare a solution of 1 part yeast broth (make sure you shake the broth) and 9 parts water. Test this solution in the calorimeter for absorbency 4. Repeat each individual test three times and take an average which you will record 5. Repeat step 2-4 three times a day (morning, afternoon, evening) for 3 days More light should be absorbed as your bacteria reproduce giving you a higher level of absorbency. Results: The two results which are missing were anomalous results caused by our research group not thoroughly shaking the yeast solution before testing it which meant that a high concentration of the yeast was at the bottom and not distributed evenly affecting the results.
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To isolate cholera from for example a fecal specimen, it is first enriched in an enrichment broth known as Alkaline peptone water or APW. it is grown in the APW for around 6 to 8 hours. It is then grown in the medium of choice which is usually Thiosulfate citrate bile salts sucrose agar or TCBS. TCBS is commonly used to isolate vibrio cholera as it specializes in isolating vibrio bacteria and is also very easy to produce and commercially available. The Aseptic technique developed to prevent contamination from unwanted microorganisms. For example when you want to set up a bacteria culture the only bacteria you would want on the petri dish is the one you are studying and therefore you would use the aseptic technique to ensure there is no contamination. We will run through how to correctly move bacteria from a broth onto a petri dish using the aseptic technique Before the procedure
The Procedure
There are two ways in which a virus can reproduce/multiply inside a cell.
There are many different ways drugs stop viruses by affecting various parts of the viruses life cycle. some of the main ones include.
Synthetic biology is a new up and coming strand of biology defined as 'designing and constructing biological devices, biological systems, and biological machines for useful purposes' this brings about a wide range of new possibilities, especially when looking into creating new 'unnatural' Amino acids. there are only 20 naturally occurring amino acids even though there are 61 codons excluding stop and start codons. scientists are therefore able to 'code' for new amino acids which can lead to genetic alterations to organism.
https://www.newscientist.com/article/dn22167-synthetic-upgrade-for-fruit-flys-dna/ This article from the New Scientist reviews scientist ability to 'hack into' the genetic code of fruit flies and use them to create new proteins by editing the amino acids and making them create some 'unnatural' amino acids. which can then be used to code for and create new proteins which in theory could be used to create organism with new or improved properties such as a "bulletproof fly" |
Jack BojanArdingly College Archives
March 2017
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