To isolate cholera from for example a fecal specimen, it is first enriched in an enrichment broth known as Alkaline peptone water or APW. it is grown in the APW for around 6 to 8 hours. It is then grown in the medium of choice which is usually Thiosulfate citrate bile salts sucrose agar or TCBS. TCBS is commonly used to isolate vibrio cholera as it specializes in isolating vibrio bacteria and is also very easy to produce and commercially available.
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Jack BojanArdingly College Archives
March 2017
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