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The graph shows us that the most effective concentration for pollen tube growth was 0.4 mol dm^3 as the percentage of pollen cells with a tube formed was 36% compared to 30% of 0.2 mol dm^3. This expreiment took place over 6 hours (09:30 - 15:30) and as we can see from our results the pollen grains in the solution containing no sucrose experienced no growth at all throughout the 6 hours period. However, the solutions with 0.8 and 1.6 mol dm^3 sucrose concertration showed relatively little growth with 8% and 2% respectively. The only difference between all the tests was the sucrose concentration that the pollen was kept in for during the experiment. Therefore differences in observations would be due to this difference in sucrose concentration. We can see that the for the higher sucrose concentrations of 1.6 and 0.8 the pollen tubes have severely stunted growth due to the fact that water travels from pollen to the surrounding through osmosis. This is due to the area surrounding the pollen being a low concentration of water. For pollen in a solution of no sucrose the pollen is in a low water concentration so, by osmosis, the water travels from the surroundings into the cytoplasm of the cell. This may end up lysing the cell if the concentration difference is significant enough. It appears, from our experiment that the concentration within the cell and the surroundings are similar enough, in the cases of 0.2 and 0.4 mol dm^3 sucrose solutions, that the pollen tubes can grow and form.
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Jack BojanArdingly College Archives
March 2017
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